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This course provides the insights and tools for the design of biotechnology processes in a sustainable way. Students will be challenged to design their own biotechnological process and evaluate its performance and sustainability. This course teaches how to apply these to the design of fermentation processes for the production of fuels, chemicals and foodstuffs, use of renewable materials and use of the unique properties of microorganisms.


This course does not involve any written exams. Students need to answer 5 assignment questions to complete the course, the answers will be in the form of written work in pdf or word. Students can write the answers in their own time. Each answer needs to be 200 words (1 Page). Once the answers are submitted, the tutor will check and assess the work.


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Course Credit: TU Delft

Course Curriculum

Module 1: Biotechnology for Biobased Products
1.1 Why develop the biobased economy? 00:08:00
1.2 Industrial biotechnology 00:06:00
1.3 Feedstocks for biobased products 00:10:00
1.4 Process to bio-PDO 00:07:00
1.5 The benefits for society 00:07:00
Best Practice 1 – Self healing concrete 00:16:00
Module 2: Balances and microbial rates
2.1 Microorganisms and their function in nature 00:09:00
2.2 Functional understanding of nutrient requirements for microbial growth 00:11:00
Banana #1 00:06:00
Banana #2 00:08:00
Banana #3 00:07:00
Banana #4 00:09:00
Best Practice 2a – Exploiting natures biodiversity 00:07:00
Best practice 2b – Application of mixed cultures 00:11:00
Module 3: The black box model and process reaction
3.1 The process reaction 00:08:00
3.2 Basics of the black box model 00:09:00
3.3 Energy consuming and energy producing products 00:11:00
3.4 A PDO black box model: experiments for parameter identification 00:08:00
3.5 Black box models: The PDO process reaction as function of mu 00:10:00
3.6 PDO continuous process design: calculation of inputs and outputs using the process reaction 00:11:00
3.7 Aerobic PDO process: improving sustainability 00:11:00
Extra guest lecture balances 00:18:00
Best Practice 3a – Industrial Process Development for Succinic Acid: Introduction 00:06:00
Best Practice 3b – Industrial Process Development for Succinic Acid: The process 00:07:00
Best Practice 3c – Industrial Process Development for Succinic Acid: Industrial scale 00:04:00
Module 4: Fermentation Design
4.1 Introduction to fermentation technology 00:07:00
4.2 The large-scale fermenter 00:08:00
4.3 Fermenter operation 00:11:00
4.4 Gas transport 00:10:00
4.5 Heat transport 00:08:00
4.6 Mixing 00:11:00
4.7 Basic approach to design and optimize a PDO fermentation process 00:12:00
Best Practice 4a – Semi-synthetic Artemisinin Production 00:09:00
Best Practice 4b – Semi-synthetic Artemisinin Production 00:08:00
Module 5: Up- and Downstream Process Integration
5.1 Towards an integrated bioprocess 00:09:00
5.2 Upstream processing 00:12:00
5.3 Separation and formulation 00:07:00
5.4 Separation principles 00:10:00
5.5 Conceptual process design for 1,3 PDO production 00:09:00
5.6 Process integration for 1,3 PDO production 00:11:00
Best Practice 5a – Bioethanol in Brazil 00:13:00
Best Practice 5b – Production of 1G bioethanol in Brazil 00:09:00
Module 6: Process Evaluation and Sustainability
6.1 Designing a sustainable business case 00:01:00
6.2 Process economics 00:11:00
6.3 Environmental assessment of a process design 00:12:00
6.4 Social assessment of a process design 00:11:00
6.5 Technology improvement 00:07:00
6.6 Sustainability in a global context 00:11:00
Best practice 6a – sustainability of 1G bioethanol in Brazil 00:11:00
Braskem’s green PE part I 00:09:00
Braskem’s green part II 00:08:00
Module 7: Closing lecture
Closing lecture 00:10:00
Submit Your Assignment 00:00:00
Certification 00:00:00

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