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If you wish to learn cryo-electron microscopy processing theory and practice with the aim of applying and developing these systems, then you’ve come to the right place.

The [course_title] is designed to introduce you to the method of cryo-electron microscopy. Usually, these methods are complicated and under constant development. Therefore, this intensive course aims to provide advanced education, which is required to assure you can adopt new technologies to expand into new areas and solve biological puzzles.

Assessment

This course does not involve any written exams. Students need to answer 5 assignment questions to complete the course, the answers will be in the form of written work in pdf or word. Students can write the answers in their own time. Each answer needs to be 200 words (1 Page). Once the answers are submitted, the tutor will check and assess the work.

Certification

Edukite courses are free to study. To successfully complete a course you must submit all the assignment of the course as part of the assessment. Upon successful completion of a course, you can choose to make your achievement formal by obtaining your Certificate at a cost of £49.

Having an Official Edukite Certification is a great way to celebrate and share your success. You can:

  • Add the certificate to your CV or resume and brighten up your career
  • Show it to prove your success

Course Credit: Open Culture

Course Curriculum

Cryo-EM Review Slides URL 00:01:00
Introduction: Why Electrons? – G. Jensen 00:09:00
Part 1: Basic Anatomy of the EM – G. Jensen 00:01:00
Part 1: Detectors – G. Jensen 00:12:00
Part 1: Electron Guns – G. Jensen 00:19:00
Part 1: EM Lenses – G. Jensen 00:32:00
Part 1: Energy Filters – G. Jensen 00:07:00
Part 1: Sample Chamber – G. Jensen 00:14:00
Part 1: Summary and Safety – G. Jensen 00:05:00
Part 1: The Column – G. Jensen 00:26:00
Part 1: Vacuum Systems – G. Jensen 00:10:00
Part 2: 1-D Reciprocal Space – G. Jensen 00:20:00
Part 2: 1-D Sine Waves and Their Sums – G. Jensen 00:33:00
Part 2: 2-D Transforms and Filters – G. Jensen 00:33:00
Part 2: 2-D Waves and Images – G. Jensen 00:19:00
Part 2: 3-D Waves and Transforms – G. Jensen 00:13:00
Part 2: Convolution and Cross-Correlation – G. Jensen 00:15:00
Part 2: Fourier Transforms for Beginners – G. Jensen 00:01:00
Part 3: Amplitude and Phase Contrast 2016 – G. Jensen 00:52:00
Part 3: CTF Correction – G. Jensen 00:32:00
Part 3: Defocus and Its Effects – G. Jensen 00:21:00
Part 3: Envelopes – G. Jensen 00:16:00
Part 3: Image Formation – G. Jensen 00:01:00
Part 3: The Contrast Transfer Function – G. Jensen 00:34:00
Part 3: Wave Propagation and Phase Shifts – G. Jensen 00:30:00
Part 4: 3-D Reconstruction – G. Jensen 00:11:00
Part 4: Dose Limitations – G. Jensen 00:22:00
Part 4: Fundamental Challenges in Biological TEM – G. Jensen 00:03:00
Part 4: Sample Prep: Grids – G. Jensen 00:11:00
Part 4: Sample Prep: Methods Involving Freezing – G. Jensen 00:28:00
Part 4: Sample Prep: Room Temperature Methods – G. Jensen 00:20:00
Part 5: Tomography Parameters and Handedness – G. Jensen 00:12:00
Part 5: Tomography Data Collection and Reconstruction – G. Jensen 00:41:00
Part 5: Tomography Identfying Objects of Interest – G. Jensen 00:25:00
Part 5: Tomography Limitations – G. Jensen 00:30:00
Part 5: Tomography Parameters and Handedness – G. Jensen 00:12:00
Part 6: Single Particle Analysis – G. Jensen 00:22:00
Part 6: SPA Data Collection – G. Jensen 00:21:00
Part 6: SPA Interpretations and Limitations – G. Jensen 00:39:00
Part 6: SPA Reconstruction Additional Topics – G. Jensen 00:44:00
Part 1: Detectors – G. Jensen 00:12:00
Getting Started in Cryo-EM with Professor Grant Jensen 00:03:00
Welcome to Cryo-EM – G. Jensen 00:08:00
Part 6: SPA Reconstruction Basic Workflow – G. Jensen 00:32:00
Part 6: SPA Interpretations and Limitations – G. Jensen 00:39:00
Part 7: 2D Crystallography Intro and Sample Prep – G. Jensen 00:06:00
Part 7: FT of a 2D Crystal – G. Jensen 00:25:00
Part 7: 2D Crystallography Data Collection and Reconstruction – G. Jensen 00:17:00
Part 7: Farewell – G. Jensen 00:03:00
Part 6: SPA Sample Prep – G. Jensen 00:23:00
Part 5: Tomography – G. Jensen 00:23:00
Part 7: Farewell – G. Jensen 00:03:00
Assessment
Submit Your Assignment 00:00:00
Certification 00:00:00

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