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If you wish to learn cryo-electron microscopy processing theory and practice with the aim of applying and developing these systems, then you’ve come to the right place.
The [course_title] is designed to introduce you to the method of cryo-electron microscopy. Usually, these methods are complicated and under constant development. Therefore, this intensive course aims to provide advanced education, which is required to assure you can adopt new technologies to expand into new areas and solve biological puzzles.
Assessment
This course does not involve any written exams. Students need to answer 5 assignment questions to complete the course, the answers will be in the form of written work in pdf or word. Students can write the answers in their own time. Each answer needs to be 200 words (1 Page). Once the answers are submitted, the tutor will check and assess the work.
Certification
Edukite courses are free to study. To successfully complete a course you must submit all the assignment of the course as part of the assessment. Upon successful completion of a course, you can choose to make your achievement formal by obtaining your Certificate at a cost of £49.
Having an Official Edukite Certification is a great way to celebrate and share your success. You can:
- Add the certificate to your CV or resume and brighten up your career
- Show it to prove your success
Course Credit: Open Culture
Course Curriculum
Cryo-EM Review Slides URL | 00:01:00 | ||
Introduction: Why Electrons? – G. Jensen | 00:09:00 | ||
Part 1: Basic Anatomy of the EM – G. Jensen | 00:01:00 | ||
Part 1: Detectors – G. Jensen | 00:12:00 | ||
Part 1: Electron Guns – G. Jensen | 00:19:00 | ||
Part 1: EM Lenses – G. Jensen | 00:32:00 | ||
Part 1: Energy Filters – G. Jensen | 00:07:00 | ||
Part 1: Sample Chamber – G. Jensen | 00:14:00 | ||
Part 1: Summary and Safety – G. Jensen | 00:05:00 | ||
Part 1: The Column – G. Jensen | 00:26:00 | ||
Part 1: Vacuum Systems – G. Jensen | 00:10:00 | ||
Part 2: 1-D Reciprocal Space – G. Jensen | 00:20:00 | ||
Part 2: 1-D Sine Waves and Their Sums – G. Jensen | 00:33:00 | ||
Part 2: 2-D Transforms and Filters – G. Jensen | 00:33:00 | ||
Part 2: 2-D Waves and Images – G. Jensen | 00:19:00 | ||
Part 2: 3-D Waves and Transforms – G. Jensen | 00:13:00 | ||
Part 2: Convolution and Cross-Correlation – G. Jensen | 00:15:00 | ||
Part 2: Fourier Transforms for Beginners – G. Jensen | 00:01:00 | ||
Part 3: Amplitude and Phase Contrast 2016 – G. Jensen | 00:52:00 | ||
Part 3: CTF Correction – G. Jensen | 00:32:00 | ||
Part 3: Defocus and Its Effects – G. Jensen | 00:21:00 | ||
Part 3: Envelopes – G. Jensen | 00:16:00 | ||
Part 3: Image Formation – G. Jensen | 00:01:00 | ||
Part 3: The Contrast Transfer Function – G. Jensen | 00:34:00 | ||
Part 3: Wave Propagation and Phase Shifts – G. Jensen | 00:30:00 | ||
Part 4: 3-D Reconstruction – G. Jensen | 00:11:00 | ||
Part 4: Dose Limitations – G. Jensen | 00:22:00 | ||
Part 4: Fundamental Challenges in Biological TEM – G. Jensen | 00:03:00 | ||
Part 4: Sample Prep: Grids – G. Jensen | 00:11:00 | ||
Part 4: Sample Prep: Methods Involving Freezing – G. Jensen | 00:28:00 | ||
Part 4: Sample Prep: Room Temperature Methods – G. Jensen | 00:20:00 | ||
Part 5: Tomography Parameters and Handedness – G. Jensen | 00:12:00 | ||
Part 5: Tomography Data Collection and Reconstruction – G. Jensen | 00:41:00 | ||
Part 5: Tomography Identfying Objects of Interest – G. Jensen | 00:25:00 | ||
Part 5: Tomography Limitations – G. Jensen | 00:30:00 | ||
Part 5: Tomography Parameters and Handedness – G. Jensen | 00:12:00 | ||
Part 6: Single Particle Analysis – G. Jensen | 00:22:00 | ||
Part 6: SPA Data Collection – G. Jensen | 00:21:00 | ||
Part 6: SPA Interpretations and Limitations – G. Jensen | 00:39:00 | ||
Part 6: SPA Reconstruction Additional Topics – G. Jensen | 00:44:00 | ||
Part 1: Detectors – G. Jensen | 00:12:00 | ||
Getting Started in Cryo-EM with Professor Grant Jensen | 00:03:00 | ||
Welcome to Cryo-EM – G. Jensen | 00:08:00 | ||
Part 6: SPA Reconstruction Basic Workflow – G. Jensen | 00:32:00 | ||
Part 6: SPA Interpretations and Limitations – G. Jensen | 00:39:00 | ||
Part 7: 2D Crystallography Intro and Sample Prep – G. Jensen | 00:06:00 | ||
Part 7: FT of a 2D Crystal – G. Jensen | 00:25:00 | ||
Part 7: 2D Crystallography Data Collection and Reconstruction – G. Jensen | 00:17:00 | ||
Part 7: Farewell – G. Jensen | 00:03:00 | ||
Part 6: SPA Sample Prep – G. Jensen | 00:23:00 | ||
Part 5: Tomography – G. Jensen | 00:23:00 | ||
Part 7: Farewell – G. Jensen | 00:03:00 | ||
Assessment | |||
Submit Your Assignment | 00:00:00 | ||
Certification | 00:00:00 |
Course Reviews
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