The course focuses on the research-inspired laboratory experience in biochemistry field. You will be introduced to the standard biochemical techniques so that you can able to investigate the present and existing research topics. Particular focus will be given on the chemical techniques of the cancer drug Gleevec. Apart from these, the course focuses on the protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.
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Course Credit: MIT
|Lec 1 Introduction. Kinases in healthy and cancerous cells||00:30:00|
|Lec 2 DNA digestion and PCR||00:35:00|
|Lec 3 Affinity tags for protein purificationdetection||00:40:00|
|Lec 4 Conserved and variable structural features of kinase domains||00:45:00|
|Lec 5 Site directed mutagenesis (Quikchange method)||00:20:00|
|Lec 6 Detecting kinase activity||00:30:00|
|Submit Your Assignment||00:00:00|
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