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The course focuses on the research-inspired laboratory experience in biochemistry field. You will be introduced to the standard biochemical techniques so that you can able to investigate the present and existing research topics. Particular focus will be given on the chemical techniques of the cancer drug Gleevec. Apart from these, the course focuses on the protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.


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Course Credit:  MIT

Course Curriculum

Lec 1 Introduction. Kinases in healthy and cancerous cells 00:30:00
Lec 2 DNA digestion and PCR 00:35:00
Lec 3 Affinity tags for protein purificationdetection 00:40:00
Lec 4 Conserved and variable structural features of kinase domains 00:45:00
Lec 5 Site directed mutagenesis (Quikchange method) 00:20:00
Lec 6 Detecting kinase activity 00:30:00
Submit Your Assignment 00:00:00
Certification 00:00:00

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